HPLC is a chromatographic technique that uses different mobile phases and columns to achieve the necessary separation of non-volatiles and other analytes that cannot be analyzed by gas chromatography. HPLC is non-destructive and analytes can be isolated for further spectroscopic analysis. Very low detection levels are achievable and multiple detectors can be used in series to monitor specific analytes. Various detectors are used in the laboratory to determine active compounds, high molecular weight analytes, and non-UV absorbing materials.
HPLC is commonly used for:
- Quantification and identification of trace organics
